本试验用水提醇沉法提取蓝刺头多糖(Echinops latifolius tausch polysaccharide,ETP),通过单因素和响应面法对ETP提取工艺进行优化,并对蓝刺头提取物进行体外抗氧化活性的测定。结果表明,蓝刺头多糖的最佳提取条件为:料液比1:20 g/mL、提取时间2 h、提取温度100 ℃。在最优条件下多糖的得率为1.191%。清除自由基结果显示,在一定浓度范围内,蓝刺头多糖对DPPH·清除率最高达93.69%,对·OH清除率最高达97.44%,对O2-·清除率最高达67.96%。研究表明,响应面对ETP的提取优化条件合理,同时保留了该多糖良好的抗氧化活性,为其临床应用提供一定的理论依据。
In this experiment,the extraction of Echinops lotifolis Tausch polysaccharides(ETP)was carried out by decoction and alcohol sedimentation technique. The extraction process of ETP was optimized by single factor experiments and response surface analysis,and the antioxidant activity in vitro was determined. The results showed that,the optimum extraction conditions for the polysaccharide were as follows:The ratio 1:20 g/mL(dry weight/water),reaction time 2 h,reaction temperature 100 ℃. The average extraction rate of polysaccharide under optimum conditions was 1.191%. The results of scavenging free radicals showed that the DPPH· clearance rate was up to 93.69%,the highest ·OH clearance rate was 97.44%,and the O2-· clearance rate was up to 67.96%. The results showed that the response to ETP was optimized. At the same time,good antioxidative activity could be obtained,which would provide a theoretical basis for its clinical application.