本研究采用响应面法优化怀菊花精油的水蒸气蒸馏提取工艺,以提高怀菊花精油得率。通过单因素实验探究加水量、蒸馏时间、浸泡时间对怀菊花精油得率的影响,并应用响应面优化提取条件。结果表明,各因素的影响重要性依次为:蒸馏时间 > 浸泡时间 > 加水量,在加水量为693 mL,蒸馏时间为8.45 h,浸泡时间为9.7 h的条件下,怀菊花精油的得率可达0.507%,与预测最佳得率0.51%存在0.59%的相对误差,且t检验的结果差异不显著,P>0.05,可以看出该模型可以较好预测实验结果,证明拟合效果良好。通过单因素与响应面法优化的怀菊花工艺条件合理可靠,为怀菊花精油的提取提供了理论依据。
The essential oil of Huai chrysanthemum was extracted by the method of steam distillation and optimized by response surface. Based on single factors including water addition amount,distillation time and soak period,the response surface method was used to optimize the extraction process conditions of Huai chrysanthemum essential oil,and the optimal extraction process conditions were explored. The results showed that the influence factors on the yield were as follows:Distillation time>soak period>water addition amount. Under the condition of 693 mL water addition amount,8.45 h of distillation and 9.7 h of immersion,the extraction rate of Huai chrysanthemum essential oil was 0.507%,whose relative error was 0.59% between predicted optimum yields 0.51%. Moreover,it was obvious to observe the model were favorable fitted with the actual value due to its t-test result which was not significant(P>0.05). The optimized extraction conditions of essential oil from Huai chrysanthemum by single factors and response surface methodology would be reasonable and reliable with certain theoretical guiding significance for the extraction of Huai chrysanthemum essential oil.