采用水提醇沉法从流苏子中提取多糖,Sevage法除蛋白,利用DEAE-52纤维和Sephadex G-100凝胶柱色谱进行分离纯化,得到多糖CRp-1,采用化学和仪器分析方法研究了多糖的总糖和蛋白质含量、单糖组成、官能团特征、空间构像等理化性质,并通过体外实验研究CRp-1对α-葡萄糖苷酶的抑制活性。结果显示,从流苏子中分离的多糖CRp-1为白色粉末,溶于水,不溶于有机溶剂;总糖含量为97.12%,蛋白质含量为1.65%,不含单糖和多酚,不属于淀粉多糖;主要由鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖和半乳糖组成,其摩尔比为1:5.381:1.146:10.804:6.953:11.908,相对分子质量分别为223200 g/mol;紫外和红外光谱显示具有多糖的特征吸收峰,且含有吡喃糖苷。CRp-1具有三股螺旋结构,且表面为三维多孔结构,并带有一定的圆形颗粒。体外α-葡萄糖苷酶抑制活性实验表明,CRp-1具有一定的α-葡萄糖苷酶抑制活性,具有浓度依赖性。
Polysaccharide CRp-1 was extracted from Chionanthus retusus Lindl.et Paxt. seed by water extraction and alcohol precipitation,protein was removed by Sevage method,and purified by DEAE-52 fiber and Sephadex G-100gel column chromatographys. The physicochemical properties,such as total sugar and protein contents,monosaccharide composition,functional group characteristics,spatial conformation were studied by chemical and instrumental analysis,and the inhibitory activity of CRp-1 on α-glucosidase was studied in vitro. The results showed that the polysaccharide CRp-1 that isolated from C. retusus seed was white powder,soluble in water and insoluble in organic solvents,the total sugar and protein contents were 97.12% and 1.65%. CRp-1 did not contain monosaccharides and polyphenols,and also not belong to starch polysaccharides. CRp-1 was mainly composed of rhamnose,arabinose,xylose,mannose,glucose and galactose,with a molar ratio of 1:5.381,1.146,10.804:6.953:11.908,and the molecular weight was 223200 g/mol. UV and IR spectra showed that there were characteristic absorption of polysaccharides and contained furanoside. CRp-1 had a triple helix structure,and the surface was a three-dimensional porous structure,with some round particles. The experiment of α-glucosidase inhibitory activity in vitro showed that CRp-1 had certain α-glucosidase inhibitory activity in a concentration-dependent manner.