为了筛选溶磷菌组合,研究多菌剂联合对辣椒生长的影响。通过菌株形态特征、生理生化和16S rRNA序列分析鉴定实验室保存的5株溶磷菌(HSYQ18、SYC3、WS54、FWG2、SCPG-7),筛选溶磷菌组合,测定了溶磷菌组合对环境因素的响应、溶磷、分泌嗜铁素和植物生长激素(IAA)等特性。同时,盆栽实验分析多菌剂组合对辣椒幼苗的生长影响。结果表明,HSYQ18为深红沙雷氏菌(Serratia rubidaea),SYC3为分散泛菌(Pantoea dispersa),WS54、FWG2和SCPG-7为假单胞菌(Pseudomonas)。溶磷菌组合WF542在Pikovskaya培养基(PKO)中连续培养96 h后无机磷浓度可达530 mg/L。溶磷菌组合WF542具有分泌嗜铁素、吲哚乙酸(IAA)的能力。溶磷菌组合WF542最适生长温度为35 ℃,低温和高温生长缓慢;pH在5.5~8.0之间都能生长较好;在1%~7% NaCl浓度之间均能较好生长,当浓度达到9%以上时基本不能生长。通过盆栽实验得知,固氮菌与WF542菌剂联合能有效促进辣椒幼苗生长。综上表明固氮菌与溶磷菌组合WF542可以作为微生物菌肥投入到农业生产中,为优良的生物肥料菌种资源利用及蔬菜的绿色生产提供重要依据。
The combination of phosphate-dissolving bacteria was screened to study the effect of combined multi-agents on the growth of pepper. 5 phosphate solubilizing bacteria(HSYQ18,SYC3,WS54、FWG2,SCPG-7)preserved in the laboratory were identified by morphological characteristics,physiological and biochemical analysis,and 16S rRNA sequence analysis,screening for phosphate-dissolving bacteria combinations,characteristics of responsing to environmental factors,solubilizing phosphorus,secreting ferritin and indole acetic acid(IAA)were tested. The results showed that HSYQ18 was Serratia rubidaea,SYC3 belonged to Pantoaea dispersa,and WS54,FWG2 and SCPG-7 were Pseudomonas. Phospholytic bacteria combination WF542 had the ability to secrete ferritin and IAA. The phosphate-solubilizing bacteria combination WF542 was cultured in Pikovskaya inorganic phosphorus(PKO)medium for 96 h and the inorganic phosphorus concentration reached 530 mg/L. The optimum growth temperature of WF542 was 35 ℃,the combination WF542 could still grow at low temperature,and high temperature slowly. The combination WF542 could grow at pH5.5~8.0,the combination WF542 grew at concentration of NaCl 1%~7%,while had little growth when the concentration was over 9%.The combination of nitrogen-fixing bacteria and WF542 could effectively promote the growth of pepper seedlings. The combination of nitrogen-fixing bacteria and phosphorus-dissolving bacteria WF542 could be used as microbial fertilizer in agricultural production,which could provide an important basis for the utilization of excellent phosphate-dissolving bacteria resources and green production of vegetables.